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Quantification of cellular properties from external fields and resulting induced velocity: Cellular hydrodynamic diameter
Author(s) -
Chalmers Jeffrey J.,
Haam Seungjoo,
Zhao Yang,
McCloskey Kara,
Moore Lee,
Zborowski Maciej,
Williams P. Stephen
Publication year - 1999
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19990905)64:5<509::aid-bit1>3.0.co;2-z
Subject(s) - settling , velocimetry , human breast , population , coulter counter , tracking (education) , mechanics , materials science , chemistry , physics , biological system , biology , cancer cell , cancer , thermodynamics , psychology , pedagogy , genetics , demography , microbiology and biotechnology , sociology
An experimental technique is discussed in which the size distribution of a population of cells is determined by calculating each cell's settling velocity. The settling velocity is determined from microscopically obtained images which were recorded on SVHS tape. These images are then computer imaged and processed, and the cell's location and velocity are determined using a computer algorithm referred to as cell tracking velocimetry (CTV). Experimental data is presented comparing the distribution of human lymphocytes and a human breast cancer cell line, MCF‐7, determined using a Coulter counter and the CTV approach. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 64: 509–518, 1999.