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Process intensification by direct product sequestration from batch fermentations: Application of a fluidised bed, multi‐bed external loop contactor
Author(s) -
Hamilton Grant E.,
Morton Philip H.,
Young Thomas W.,
Lyddiatt Andrew
Publication year - 1999
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19990805)64:3<310::aid-bit7>3.0.co;2-j
Subject(s) - fermentation , chemistry , yield (engineering) , chromatography , contactor , product inhibition , yeast , bioproducts , adsorption , yarrowia , bioreactor , pulp and paper industry , biochemistry , microbiology and biotechnology , materials science , biology , organic chemistry , enzyme , biofuel , non competitive inhibition , metallurgy , engineering , power (physics) , physics , quantum mechanics
A critical comparison has been made of the relative efficacy of the primary purification of an extracellular acid protease produced by the yeast Yarrowia lipolytica. The performance of conventional, discrete sequences of fermentation, broth clarification and fixed bed, anion exchange chromatography has been compared with fluidised bed adsorption directly interfaced with post‐term fermentation broth and fluidised bed adsorption directly integrated with productive fermentations (so‐called direct product sequestration; DPS). Advantages of the latter, in terms of the improved yield and molecular quality of the protease end product are discussed in terms of the design, assembly and operation of component parts of DPS devices and their generic application to other extracellular bioproducts of microbial fermentations. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 64: 310–321, 1999.