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Recombinant protein synthesis in Trichoplusia ni BTI‐Tn‐5B1‐4 insect cell aggregates
Author(s) -
Saarinen Mark A.,
Troutner Kimberly A.,
Gladden Steve G.,
MitchellLogean Christine M.,
Murhammer David W.
Publication year - 1999
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19990605)63:5<612::aid-bit11>3.0.co;2-c
Subject(s) - trichoplusia , spodoptera , recombinant dna , biology , cell culture , microbiology and biotechnology , cell , biochemistry , gene , botany , lepidoptera genitalia , noctuidae , genetics
The Trichoplusia ni BTI‐Tn‐5B1‐4 (Tn‐5B1‐4) insect cell line has received considerable attention as a host for the baculovirus expression vector system. In the present study, suspension cultures were used to compare Tn‐5B1‐4 cell aggregates and cells selected to grow predominantly as individual cells. No significant difference was found between cell aggregates and cells growing predominantly individually in regard to cell growth rate, glucose consumption and lactate accumulation, and specific recombinant protein synthesis levels. In addition, the levels of recombinant protein synthesis were considerably higher than those produced by the commonly used Spodoptera frugiperda Sf‐9 insect cell line. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 63: 612–617, 1999.