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Integrated reversed micellar extraction and stripping of α‐amylase
Author(s) -
Lazarova Z.,
Tonova K.
Publication year - 1999
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19990605)63:5<583::aid-bit8>3.0.co;2-u
Subject(s) - chemistry , stripping (fiber) , pulmonary surfactant , extraction (chemistry) , micelle , ammonium bromide , chromatography , aqueous solution , cationic polymerization , amylase , ammonium , chemical engineering , enzyme , organic chemistry , biochemistry , materials science , engineering , composite material
In this work the possibility of integrating the two process steps—extraction of an enzyme from its source aqueous solution into an organic solvent containing reversed micelles and its stripping into another aqueous medium of appropriate composition is studied. The kinetics of enzyme mass transfer during the integrated process is investigated using a stirred cell with two separated compartments. The enzyme/surfactant system chosen consisted of α‐amylase and the cationic surfactant CTAB (cetyl trimethyl ammonium bromide). Comparison between the classical extraction process and the “integrated reversed micellar extraction–stripping” is made. The enzyme activity changes during the transfer process are followed, and the registered tendencies are discussed. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 63: 583–592, 1999.