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Enhancement of enzyme reaction of magnetically anisotropic polyacrylamide gel rods immobilized with ferromagnetic powder and β‐ D ‐galactosidase in an alternating magnetic field
Author(s) -
Sakai Yasuzo,
Oishi Ayumi,
Takahashi Fujio
Publication year - 1999
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19990205)62:3<363::aid-bit13>3.0.co;2-8
Subject(s) - rod , ferromagnetism , magnetic field , materials science , polyacrylamide , polyacrylamide gel electrophoresis , silica gel , nuclear magnetic resonance , analytical chemistry (journal) , magnetostatics , substrate (aquarium) , chemistry , composite material , chromatography , condensed matter physics , enzyme , polymer chemistry , physics , medicine , alternative medicine , oceanography , pathology , quantum mechanics , geology
An immobilized polyacrylamide gel containing β‐ D ‐galactosidase and Sr–Ba–ferrite was magnetized in a static magnetic field. The gel rods (10 mm long, Ø 2 mm) exhibiting magnetic anisotropy could move at lower than 100 Hz but not at higher than 250 Hz in an alternating magnetic field of 200 Oe. In case of immovability of gel rods, the apparent enzymic activity increased 3 times higher under exposure of an alternating magnetic field of 500 Oe (570 Hz). It could be explained that the ferromagnetic powder inside the gel might vibrate under the influence of elasticity of gel in the alternating magnetic field of 100 or 500 Oe and 0.2–12 kHz. This might facilitate faster diffusion of the substance inside the gel and transportation of the substrate and the product through the surface of gel. Consequently, the enzyme reaction was apparently activated. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 62: 363–367, 1999.