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Time Course of SDS–alkaline lysis of recombinant bacterial cells for plasmid release
Author(s) -
Ciccolini L. A. S.,
Shamlou P. Ayazi,
TitchenerHooker N. J.,
Ward J. M.,
Dunnill P.
Publication year - 1998
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19981220)60:6<768::aid-bit13>3.0.co;2-d
Subject(s) - alkaline lysis , recombinant dna , plasmid , lysis , microbiology and biotechnology , chemistry , bacteria , biochemistry , biology , dna , gene , genetics , dna vaccination
SDS–alkaline lysis of recombinant Escherichia coli cell suspensions was carried out in a coaxial cylinder rheometer, and the data were used to establish the time course of lysis reaction. The results of the experiments showed that cell lysis reaction time depended on cell strain but was unaffected by plasmid size and plasmid copy number. The high molecular weight globular proteins and chromosomal DNA were denatured, and the resulting changes in rheometric measurements characterised the denaturation time. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 60: 768–770, 1998.