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Transient studies of light‐adapted cultures of hairy roots of Catharanthus roseus: Growth and indole alkaloid accumulation
Author(s) -
Bhadra Rajiv,
Morgan John A.,
Shanks Jacqueline V.
Publication year - 1998
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19981220)60:6<670::aid-bit4>3.0.co;2-j
Subject(s) - indole alkaloid , alkaloid , catharanthus roseus , vindoline , indole test , botany , chemistry , biology , biochemistry , genetics , chemotherapy , vinblastine
Cultures of C. roseus transgenic (“hairy”) root clones LBE‐6‐1 and LBE‐4‐2 were adapted with periodic daily illumination to investigate the effect of light on growth and nutrient utilization, and the accumulation of the indole alkaloids. Light‐adapted roots appeared green and had radially thickened morphology compared with dark‐grown controls. Their growth rates were higher than dark‐grown controls, with 45% lower doubling times: LBE‐6‐1, 3.6 days; LBE‐4‐2, 2.8 days. Relative to dark‐grown controls, light‐adapted growth increased the biomass (DW) of LBE‐6‐1 by 25%, but had no effect on the DW of LBE‐4‐2. The macronutrients NH 4 + , NO 3 − , P i , and sugars, were depleted completely by light‐adapted root cultures in that order. The specific and total levels of the indole alkaloid serpentine was enhanced and of tabersonine was lowered in both root clones, while the overall trends of growth and non‐growth association of tabersonine and serpentine, respectively, remained unaltered by light adaptation. Ajmalicine accumulation was enhanced in LBE‐6‐1, but lowered in LBE‐4‐2; its accumulation was growth‐associated in dark‐grown LBE‐6‐1, but appeared non‐growth associated in light‐adapted cultures. The accumulation of tabersonine‐related compounds, lochnericine, and hörhammericine exhibited growth‐associated trends, and were either negatively affected or unaffected by light adaptation of LBE‐6‐1. Neither vindoline nor its precursor, deacetylvindoline, was detected. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 60: 670–678, 1998.