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The expression of recombinant genes from bacteriophage lambda strong promoters triggers the SOS response in Escherichia coli
Author(s) -
Arís A.,
Corchero J. L.,
Benito A.,
Carbonell X.,
Viaplana E.,
Villaverde A.
Publication year - 1998
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19981205)60:5<551::aid-bit5>3.0.co;2-m
Subject(s) - bacteriophage , recombinant dna , gene , escherichia coli , lambda , promoter , biology , microbiology and biotechnology , genetics , gene expression , physics , optics
The production of several non‐related heterologous proteins in recombinant Escherichia coli cells promotes a significant transcription of recA and sfiA SOS DNA repair genes. The activation of the SOS system occurs when the expression of plasmid‐encoded genes is directed by the strong lambda lytic promoters, but not by IPTG‐controlled promoters either at 37 or at 42°C, and it is linked to an extensive degradation of the proteins after their synthesis. The triggering signal for the SOS response could be an important arrest of cell DNA replication observed within the first hour after the induction of recombinant gene expression. The stimulation of this DNA repair system can partially account for the toxicity exhibited by recombinant proteins on actively producing E. coli cells. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 60: 551–559, 1998.