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Fed‐batch production of Thermomonospora fusca endoglucanase by recombinant Streptomyces lividans
Author(s) -
Kim Eunki,
Shin DongHoon,
Irwin Diana C.,
Wilson David B.
Publication year - 1998
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19981005)60:1<70::aid-bit8>3.0.co;2-y
Subject(s) - cellulase , fermentation , aeration , chemistry , ammonium sulfate , biochemistry , streptomycetaceae , food science , biology , actinomycetales , hydrolysis , streptomyces , chromatography , bacteria , organic chemistry , genetics
Abstract The factors affecting the production of a Thermomonospora fusca endoglucanase by a recombinant Streptomyces lividans strain were studied in a fermentor with glucose addition controlled by a pH‐stat. The recombinant plasmid was stable for 35 generations with constant endoglucanase productivity. Glucose and peptone were used as the carbon and nitrogen sources. Addition of Tween‐80 increased endoglucanase production twofold. A significant decrease in endoglucanase production was observed at low aeration. During fed‐batch cultivation, pulse feeding (6 g/L) of a glucose‐ammonium sulfate solution was optimal for endoglucanase production. With higher concentrations of glucose (15 g/L), a significant amount of organic acid, including acetic acid, was produced, which inhibited cell growth and endoglucanase production. Under optimum conditions, 1.7 U/mL of endoglucanase were produced. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 60: 70–76, 1998.