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Two‐coat systems for encapsulation of Spathoglottis plicata (Orchidaceae) seeds and protocorms
Author(s) -
Khor Eugene,
Ng WengFun,
Loh ChiangShiong
Publication year - 1998
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19980905)59:5<635::aid-bit14>3.0.co;2-8
Subject(s) - desiccation , germination , orchidaceae , coat , abscisic acid , biology , horticulture , botany , ecology , biochemistry , gene
Complex coacervation of alginate–chitosan and alginate–gelatin were used to develop two‐coat systems for the encapsulation of Spathoglottis plicata seeds and protocorms (top‐shaped structures formed after seed germination of orchids). Both the seeds and the protocorms could withstand the encapsulation treatments with high viability. About 54% of seeds and 40% of large protocorms (1.6–2.0 mm) were able to tolerate a 6‐h desiccation treatment. However, viability of the small protocorms (0.7–0.9 mm) was greatly reduced if they were desiccated before encapsulation. Encapsulation after desiccation significantly increased the percentage viability of seeds and protocorms. Treatment with abscisic acid (ABA, 10 −5 M ) before desiccation and encapsulation resulted in high percentage viability in seeds and large protocorms whereas the small protocorms were found to be less tolerant to the treatments. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 59:635–639, 1998.