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Dynamic modeling of meta ‐ and para ‐nitrobenzoate metabolism by a mixed co‐immobilized culture of Comamonas spp. JS46 and JS47
Author(s) -
Goodall Jennifer L.,
Peretti Steven W.
Publication year - 1998
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19980820)59:4<507::aid-bit14>3.0.co;2-6
Subject(s) - respirometry , biomass (ecology) , substrate (aquarium) , chemistry , transient (computer programming) , kinetics , bioreactor , effluent , metabolic activity , biological system , metabolism , biochemical engineering , chemical engineering , biochemistry , environmental science , biology , ecology , environmental engineering , computer science , organic chemistry , physics , operating system , engineering , quantum mechanics
A model describing the transient activity of a mixed immobilized culture of Comamonas spp. JS46 and JS47 growing on mixed substrates is presented. The transient periods considered are those following changes in the feed carbon source, which alternated between meta ‐ and para ‐nitrobenzoate. The feed profile alternately starved one of the species in the mixed culture. The response of the system, as quantified by the reactor effluent substrate concentrations, is dictated by the activity of the biomass and the appropriate biochemical pathway. As detailed mechanistic pathway information is not available, respirometry has been used to characterize both facets of activity. Two parameters were introduced: Ψ representing pathway activity and Γ representing biomass activity; a detailed description of the analysis is included. The model is compared to experimental investigation of the system and describes the reactor response well. The agreement between model and experiment suggests the usefulness of oxygen kinetics as global measurements to describe complex systems when mechanistic detail is not available. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 59: 507–516, 1998.