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Improvement of heterologous protein productivity using recombinant Yarrowia lipolytica and cyclic fed‐batch process strategy
Author(s) -
Chang Ching Chuan,
Ryu Dewey D. Y.,
Park Cheon Seok,
Kim JeongYoon
Publication year - 1998
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19980805)59:3<379::aid-bit15>3.0.co;2-9
Subject(s) - bioprocess , heterologous , yarrowia , bioprocess engineering , fed batch culture , bioreactor , productivity , biology , growth rate , microbiology and biotechnology , biochemistry , yeast , fermentation , botany , mathematics , gene , paleontology , geometry , macroeconomics , economics
A cyclic fed‐batch bioprocess is designed and a significant improvement of rice α‐amylase productivity of recombinant Yarrowia lipolytica is illustrated. A bioprocess control strategy developed and reported here entails use of a genetically stable recombinant cloned for heterologous protein, use of optimized media for cell growth and enzyme production phases, and process control strategy enabling high cell‐density culture and high α‐amylase productivity. This process control can be achieved through maintaining a constant optimal specific cell growth rate at a predetermined value (i.e., 0.1 h −1 ), controlling medium feed rate commensurate with the cell growth rate, and maintaining a high cell‐density culture (i.e., 60–70 g/L) for high productivity of cloned heterologous protein. The volumetric enzyme productivity (1,960 units/L · h) achieved from the cyclic fed‐batch process was about 3‐fold higher than that of the fed‐batch culture process (630 units/L · h). © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 59:379–385, 1998.