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Theoretical analysis of cell separation based on cell surface marker density
Author(s) -
Chalmers Jeffrey J.,
Zborowski Maciej,
Moore Lee,
Mandal Sushim,
Fang BingBing,
Sun Liping
Publication year - 1998
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19980705)59:1<10::aid-bit3>3.0.co;2-w
Subject(s) - cell , chemistry , chromatography , population , surface (topology) , adsorption , biophysics , analytical chemistry (journal) , biology , biochemistry , mathematics , geometry , demography , organic chemistry , sociology
A theoretical analysis was performed to determine the number of fractions a multidisperse, immunomagnetically labeled cell population can be separated into based on the surface marker (antigen) density. A number of assumptions were made in this analysis: that there is a proportionality between the number of surface markers on the cell surface and the number of immunomagnetic labels bound; that this surface marker density is independent of the cell diameter; and that there is only the presence of magnetic and drag forces acting on the cell. Due to the normal distribution of cell diameters, a “randomizing” effect enters into the analysis, and an analogy between the “theoretical plate” analysis of distillation, adsorption, and chromatography can be made. Using the experimentally determined, normal distribution of cell diameters for human lymphocytes and a breast cancer cell line, and fluorescent activated cell screening data of specific surface marker distributions, examples of theoretical plate calculations were made and discussed. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 59:10–20, 1998.