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A novel biotinylated degradable polymer for cell‐interactive applications
Author(s) -
Cannizzaro Scott M.,
Padera Robert F.,
Langer Robert,
Rogers Rick A.,
Black Fiona E.,
Davies Martyn C.,
Tendler Saul J. B.,
Shakesheff Kevin M.
Publication year - 1998
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19980605)58:5<529::aid-bit9>3.0.co;2-f
Subject(s) - biotinylation , polymer , ethylene glycol , surface plasmon resonance , cell adhesion , adhesion , streptavidin , avidin , biophysics , peg ratio , tissue engineering , materials science , copolymer , biomolecule , surface modification , chemistry , nanotechnology , biotin , nanoparticle , biochemistry , biomedical engineering , organic chemistry , medicine , finance , economics , biology
We describe the development of a novel biodegradable polymer designed to present bioactive motifs at the surfaces of materials of any architecture. The polymer is a block copolymer of biotinylated poly(ethylene glycol) (PEG) with poly(lactic acid) (PLA); it utilizes the high‐affinity coupling of the biotin–avidin system to undergo postfabrication surface engineering. We show, using surface plasmon resonance analysis (SPR) and confocal microscopy that surface engineering can be achieved under aqueous conditions in short time periods. These surfaces interact with cell surface molecules and generate beneficial responses as demonstrated by the model study of integrin‐mediated spreading of endothelial cells on polymer surfaces presenting RGD peptide adhesion sequences. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 58: 529–535, 1998.