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Removal of nitrate from industrial wastewaters in a pilot plant by nitrate‐tolerant Klebsiella oxytoca CECT 4460 and Arthrobacter globiformis CECT 4500
Author(s) -
Piñar Guadalupe,
Oliva JoséMiguel,
SánchezBarbero Luis,
Calvo Victor,
Ramos Juan L.
Publication year - 1998
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19980605)58:5<510::aid-bit7>3.0.co;2-g
Subject(s) - nitrate , klebsiella oxytoca , nitrite , effluent , chemistry , arthrobacter , bacteria , industrial waste , ammonium , microbiology and biotechnology , pulp and paper industry , environmental engineering , biology , environmental science , enterobacteriaceae , ecology , biochemistry , escherichia coli , genetics , organic chemistry , gene , engineering
Two strains, a gram‐negative bacterium Klebsiella oxytoca CECT 4460 and a gram‐positive, mycelium‐forming bacterium Arthrobacter globiformis CECT 4500, tolerant to up to 1 M nitrate, were isolated from the grounds of a munitions factory. Under strict aerobic conditions and with appropriate C‐sources, growth of these bacteria took place when the nitrate concentration in the medium was below 150 m M. Optimal growth conditions regarding the culture medium composition for the biological removal of nitrate were established in batch cultures. Then, the system was scaled up to a 40‐L pilot plant and operated under continuous conditions in a factory with direct waste streams from dinitroethylene glycol production after appropriate dilution with nontreated groundwaters. The level of nitrate in the effluent was below 0.5% of the initial N‐load. Nitrite and ammonium were undetectable and the level of the C‐source in the effluent was below 50 mg per L. On the basis of these results, we conclude that the system worked on site satisfactorily. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 58: 510–514, 1998.