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Factorial optimization of a six‐cellulase mixture
Author(s) -
Kim Eunki,
Irwin Diana C.,
Walker Larry P.,
Wilson David B.
Publication year - 1998
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19980605)58:5<494::aid-bit5>3.0.co;2-8
Subject(s) - cellulase , trichoderma reesei , yield (engineering) , factorial experiment , chemistry , filter paper , enzyme , chromatography , food science , biochemistry , mathematics , materials science , statistics , metallurgy
A factorial experimental design approach was used to optimize mixtures of six cellulases (five Thermomonospora fusca cellulases and plus/minus Trichoderma reesei CBHI along with β‐glucosidase) so as to maximize the glucose produced from filter paper. Optimized mixture A and mixture B produced glucose at 25 and 8.3 μmol glucose/μmol enzyme/min, respectively, which are 8 and 1.5 times higher than the sum of the activity of the individual cellulases. In both mixtures, the glucose yield depended on the ratio and the cellulases used. Most enzymes showed synergistic interactions that increased the glucose yield. The yield of glucose with the optimum mixtures depended on the total enzyme concentration. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 58: 494–501, 1998.