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High cell density culture of metabolically engineered Escherichia coli for the production of poly(3‐hydroxybutyrate) in a defined medium
Author(s) -
Wang Fulai,
Lee Sang Yup
Publication year - 1998
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19980420)58:2/3<325::aid-bit33>3.0.co;2-8
Subject(s) - escherichia coli , filamentation , recombinant dna , ftsz , polyhydroxybutyrate , polyhydroxyalkanoates , plasmid , strain (injury) , biochemistry , enterobacteriaceae , biology , fed batch culture , microbiology and biotechnology , biosynthesis , bacteria , fermentation , chemistry , gene , genetics , laser , physics , optics , anatomy
A recombinant Escherichia coli strain XL1‐Blue harboring a stable high‐copy‐number plasmid pSYL107 containing the Alcaligenes eutrophus polyhydroxyalkanoate biosynthesis genes and the Escherichia coli ftsZ gene was employed for the production of poly(3‐hydroxybutyrate) (PHB) by fed‐batch culture in a defined medium. Suppression of filamentation by overexpressing the cell division protein FtsZ allowed production of PHB to a high concentration (77 g/L) with high productivity (2 g/L/h) in a defined medium, which was not possible with the recombinant E. coli that underwent filamentation. Further optimization of fed‐batch culture condition resulted in PHB concentration of 104 g/L in a defined medium, which was the highest value reported to date by employing recombinant E. coli. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 58:325–328, 1998.