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Polysaccharide production by plant cells in suspension: experiments and mathematical modeling
Author(s) -
Glicklis R.,
Mills D.,
Sitton D.,
Stortelder W.,
Merchuk J. C.
Publication year - 1998
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19980320)57:6<732::aid-bit10>3.0.co;2-9
Subject(s) - erlenmeyer flask , laboratory flask , suspension culture , extracellular , dry weight , suspension (topology) , polysaccharide , substrate (aquarium) , biomass (ecology) , intracellular , chemistry , extracellular polysaccharide , botany , chromatography , food science , biology , cell culture , biochemistry , mathematics , agronomy , ecology , organic chemistry , homotopy , pure mathematics , genetics
Symphytum officinale L cells were grown in Erlenmeyer flasks at four different temperatures: 15, 20, 25, and 30°C. A mathematical model of the culture growth is presented. The intracellular and extracellular products are considered in separate equations. An interrelation between fresh weight, dry weight, and viability is considered in the balances. The model includes a description of the changes in time of wet and dry biomass, cell viability, substrate concentration and polysaccharide concentration, both intra‐ and extracellular. The model was tested by fitting the numerical results to the data obtained. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 57: 732–740, 1998