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Optimization of Phaffia rhodozyma continuous culture through response surface methodology
Author(s) -
Vázquez Manuel,
Martin Antonio M.
Publication year - 1998
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19980205)57:3<314::aid-bit8>3.0.co;2-k
Subject(s) - dilution , biomass (ecology) , response surface methodology , astaxanthin , fermentation , substrate (aquarium) , factorial experiment , chemistry , yield (engineering) , yeast , chromatography , fractional factorial design , food science , biochemistry , mathematics , biology , materials science , agronomy , thermodynamics , ecology , carotenoid , physics , statistics , metallurgy
Response surface methodology was applied to optimize the growth of the yeast Phaffia rhodozyma in continuous fermentation using peat hydrolysates as substrate. A second‐order, complete, factorial design of the experiments was used to develop empirical models providing a quantitative interpretation of the relationships between the two variables studied, dilution rate and pH. Maximum biomass concentration in the fermentor was obtained by employing the following predicted optimum fermentation conditions: a dilution rate of 0.017/h and a pH level of 7.19. A verification experiment, conducted at previously optimized conditions for maximum biomass volumetric productivity (a dilution rate of 0.022/h, and a pH level of 6.90), produced values for biomass concentration, residual substrate concentration, biomass yield, and biomass volumetric productivity that were very close to the predicted values, indicating the reliability of the empirical model. The concentration of the pigment astaxanthin produced by the yeast under the optimized growth conditions was found to be 544 mg astaxanthin/kg dry cell biomass. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 57: 314–320, 1998.