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Effect of salt hydrate pair on lipase‐catalyzed regioselective monoacylation of sucrose
Author(s) -
Kim Jae Eun,
Han Jeong Jun,
Yoon Jung Hae,
Rhee Joon Shick
Publication year - 1998
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19980105)57:1<121::aid-bit15>3.0.co;2-n
Subject(s) - chemistry , lipase , sucrose , maltose , fructose , yield (engineering) , organic chemistry , lactose , salt (chemistry) , capric acid , triacylglycerol lipase , fatty acid , lauric acid , enzyme , materials science , metallurgy
Sucrose monoesters of a fatty acid were synthesized by using lipase in a solvent‐free system. When lipase from Mucor miehei was used as a catalyst with capric acid as the donor and sugar as the acceptor, sucrose 6‐monocaprate was predominantly produced in a yield of 25.3%. The yield of product was significantly increased by the direct addition of a suitable pair of solid salt hydrates to the reaction mixture to control the water activity ( a w ). Among the salt hydrate pairs investigated, the barium hydroxide, 8/1H 2 O pair resulted in the highest yield of the product. This salt addition method was also successfully employed for acylation of primary hydroxyl groups in various unprotected mono‐ and disaccharides such as glucose, galactose, fructose, trehalose, mannose, maltose, and lactose. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 57: 121–125, 1998.