Premium
Monitoring solution‐phase combinatorial library synthesis by capillary electrophoresis
Author(s) -
Gaus Hans J.,
Kung PeiPei,
Brooks Doug,
Cook P. Dan,
Cummins Lendell L.
Publication year - 1999
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(1998)61:3<169::aid-cc4>3.0.co;2-6
Subject(s) - capillary electrophoresis , chemistry , chromatography , alkylation , electrophoresis , halide , phase (matter) , capillary action , aqueous solution , combinatorial chemistry , analytical chemistry (journal) , organic chemistry , materials science , composite material , catalysis
Capillary electrophoresis has been applied to monitor model reactions in solution‐phase combinatorial chemistry. In particular, the simultaneous alkylation reactions of secondary amines with a series of benzyl halides has been investigated. Reactant and product concentrations were monitored using capillary electrophoresis in a non‐aqueous buffer system. The simplified sample preparation was a key feature making this an attractive method of analysis. The results demonstrate that capillary electrophoresis is a useful tool for monitoring reactions to determine initial rates, rate constants, and extinction correlation coefficients for quantitative analysis in combinatorial chemistry, and is a broadly applicable technique for the analysis of a variety of organic and bioorganic transformations. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng (Comb Chem) 61:169–177, 1998/1999.