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Ammonium toxicity in different cell lines
Author(s) -
Mirabet Maribel,
Navarro Angels,
Lopez Anna,
Canela Enric I.,
Mallol Josefa,
Lluis Carmen,
Franco Rafael
Publication year - 1997
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19971205)56:5<530::aid-bit6>3.0.co;2-j
Subject(s) - jurkat cells , ammonium , intracellular , programmed cell death , cell culture , apoptosis , toxicity , chemistry , cell , biology , microbiology and biotechnology , biochemistry , biophysics , t cell , immunology , immune system , genetics , organic chemistry
The toxic effect of ammonium upon a variety of cell lines of lymphoid (Jurkat), pituitary (GH 4 ), and renal (LLC‐PK 1 ) origin was studied. Millimolar concentrations of the ion mildly affected the growth of GH 4 cells and prevented the growth of LLC‐PK 1 cells. The ion did not lead to the death of LLC‐PK 1 cells but it produced morphologic changes in these cells. The effects of ammonium upon Jurkat cells were different because cells died after accumulating at S phase. Cell death was due to apoptosis and might be related to ammonium‐induced calcium mobilization from intracellular stores. These results indicate that the toxic effects caused by ammonium accumulation are different depending upon the cell type. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 56: 530–537, 1997.