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Experimental investigation and modeling of oscillatory behavior in the continuous culture of Zymomonas mobilis
Author(s) -
Daugulis Andrew J.,
McLellan P. James,
Li Jinghong
Publication year - 1997
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19971005)56:1<99::aid-bit11>3.0.co;2-5
Subject(s) - zymomonas mobilis , fermentation , oscillation (cell signaling) , biological system , biochemical engineering , lag , ethanol , chemistry , process (computing) , control theory (sociology) , computer science , ethanol fuel , biology , biochemistry , engineering , artificial intelligence , computer network , control (management) , operating system
The mechanism causing oscillation in continuous ethanol fermentation by Zymomonas mobilis under certain operating conditions has been examined. A new term, “dynamic specific growth rate,” which considers inhibitory culture conditions in the recent past affecting subsequent cell behavior, is proposed in this article. Based on this concept, a model was formulated to simulate the oscillatory behavior in continuous fermentation of Zymomonas mobilis. Forced oscillation fermentation experiments, in which exogenous ethanol was added at a controlled rate to generate oscillatory behavior, were performed in order to obtain estimates for the model parameters and to validate the proposed model. In addition, data from a literature example of a sustained oscillation were analyzed by means of the model, and excellent agreement between the model simulation and experimental results was obtained. The lag in the cells' response to a changing environment, i.e., ethanol concentration change rate experienced by the cells, was shown to be the major factor contributing to the oscillatory behavior in continuous fermentation of Zymomonas mobilis under certain operating conditions. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 56: 99–105, 1997.