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Kinetic studies on hybridoma cells immobilized in fixed bed reactors
Author(s) -
Pörtner Ralf,
Rössing Sabine,
Koop Matthias,
Lüdemann Ines
Publication year - 1997
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19970805)55:3<535::aid-bit10>3.0.co;2-f
Subject(s) - substrate (aquarium) , bioreactor , chemistry , chromatography , volume (thermodynamics) , metabolite , glutamine , dilution , volumetric flow rate , oxygen , monoclonal antibody , biophysics , perfusion , biochemistry , biology , thermodynamics , antibody , medicine , amino acid , ecology , organic chemistry , immunology , physics
Cultures with immobilized hybridoma cells were performed in fixed bed systems. “Steady state” values for volume‐specific substrate uptake and metabolite production rates were determined at various perfusion rates and superficial flow velocities of the medium within the carrier matrix. Data from fixed bed volumes between 50 and 600 ml did not show any difference. The volume‐specific glutamine and glucose uptake rate turned out to be independent of the superficial flow velocity, but decreased with decreasing glutamine and glucose concentration. The volume‐specific oxygen uptake rate increased with increasing superficial flow velocity and substrate concentration, respectively. A similar behavior was observed for the ratio between oxygen and glucose uptake rate. The production rate for monoclonal antibodies was neither affected by the substrate concentration nor by the superficial flow velocity. The metabolic parameters of the immobilized cells were put into kinetic equations and compared to those of suspended cells. It could be concluded that the metabolism of the immobilized cells is determined by the oxygen supply within the macroporous carriers. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 55 : 535–541, 1997.