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Novel preparation method for surfactant‐lipase complexes utilizing water in oil emulsions
Author(s) -
Okazaki ShinYa,
Kamiya Noriho,
Abe Kojiro,
Goto Masahiro,
Nakashio Fumiyuki
Publication year - 1997
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19970720)55:2<455::aid-bit23>3.0.co;2-e
Subject(s) - lipase , pulmonary surfactant , lauric acid , chemistry , triacylglycerol lipase , microemulsion , organic chemistry , chromatography , aqueous solution , polyvinyl alcohol , enzyme , biochemistry , fatty acid
A novel preparation method for surfactant–lipase complexes has been developed utilizing water in oil emulsions. In order to optimize the preparation conditions, we have investigated the effects of several operational parameters on the enzymatic activity of the surfactant‐lipase complexes in organic media. When a nonionic surfactant was employed under optimal preparation conditions [alkaline pH 8–10, organic/aqueous = 90/10 (v/v), concentration of surfactant, 10 m M [, the surfactant‐lipase complex efficiently catalyzed the esterification of benzyl alcohol with lauric acid in organic media. The esterification rate of the surfactant‐lipase complex was increased over 16‐fold relative to the native powder lipase. Furthermore, the lipase complex showed high storage stability. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 55 : 455–460, 1997.