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Biomass measurement by inductive permittivity
Author(s) -
Siano Steven A.
Publication year - 1997
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19970720)55:2<289::aid-bit7>3.0.co;2-e
Subject(s) - permittivity , relative permittivity , electrode , polarization (electrochemistry) , materials science , interference (communication) , analytical chemistry (journal) , chemistry , dielectric , optoelectronics , chromatography , channel (broadcasting) , electrical engineering , engineering
An electrodeless, inductive permittivity probe is shown to reliably indicate fermentation biomass. By designing the probe based on electromagnetic induction, the electrode polarization artifact—common to electrode‐based permittivity measurements of conductive solutions—is eliminated. The measurement of viable biomass concentration ( X V ) is precise and linear, with negligible interference, as shown for two bacteria, two yeasts, and two mammalian cells. The permittivity spectra often can be described by the three parameters of the Cole–Cole equation: the maximum (low‐frequency) permittivity, the characteristic frequency ( f C ), and the Cole–Cole α. The f C and α have potential as relative indicators of cell size and morphology, respectively. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 55 : 289–304, 1997