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Influence of the nature of modifier in the enzymatic activity of chemical modified semipurified lipase from Candida rugosa
Author(s) -
Hernáiz M. J.,
SánchezMontero J. M.,
Sinisterra J. V.
Publication year - 1997
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19970720)55:2<252::aid-bit2>3.0.co;2-h
Subject(s) - tetranitromethane , candida rugosa , lipase , chemistry , chemical modification , organic chemistry , cyanuric chloride , peg ratio , polyethylene glycol , hydrolysis , triacylglycerol lipase , chemical stability , enzyme , biochemistry , finance , economics
Semipurified lipase of Candida rugosa (CRSL) was subjected to chemical modification, and the activities of the modified lipase, in hydrolysis and esterification reactions, were examined. The esterification reactions were carried out in the absence and presence of isooctane. When the enzyme was modified with polyethylene glycol (PEG), two methodologies were studied. The activation of PEG with p ‐NO 2 ‐phenylchloroformate gives better biocatalysts than those obtained with cyanuric chloride‐PEG. The chemical modification with PEG increases the stability of pure lipases in isooctane at 50°C (extreme conditions). The chemically modified enzymes are useful for biotransformations in organic solvents. In addition the nitration of tyrosines with tetranitromethane was also studied. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 55 : 252–260, 1997.