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Production of actinorhodin by Streptomyces coelicolor A3(2) grown in chemostat culture
Author(s) -
Melzoch Karel,
de Mattos M. Joost Teixeira,
Neijssel Oense M.
Publication year - 1997
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19970620)54:6<577::aid-bit8>3.0.co;2-g
Subject(s) - actinorhodin , streptomyces coelicolor , chemostat , biology , streptomyces , bacteria , genetics
Streptomyces coelicolor was grown in variously limited chemostat cultures and the specific rate of extracellular actinorhodin production (q actinorhodin ) was measured. The highest q actinorhodin values were observed in glucose‐ or ammonia‐limited cultures, whereas almost no actinorhodin was produced in sulfate‐, phosphate‐, potassium‐, or magnesium‐limited cultures. The effect of the dilution rate on actinorhodin production was studied in glucose‐limited cultures. It was found that q actinorhodin was highest at D = 0.06h −1 , which was well below the maximal D value tested (0.14 h −1 ). This explains why, in batch cultures, actinorhodin production starts at the onset of the stationary phase. It was also found that the use of nitrilotriacetate instead of citrate as a chelating agent had a negative effect on actinorhodin production. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 53: 577–582, 1997.