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Kinetics and response of a Pseudomonas fuorescens HK44 biosensor
Author(s) -
Webb O. F.,
Bienkowski P. R.,
Matrubutham U.,
Evans F. A.,
Heitzer A.,
Sayler G. S.
Publication year - 1997
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19970605)54:5<491::aid-bit8>3.0.co;2-9
Subject(s) - bioreporter , pseudomonas fluorescens , reporter gene , chemistry , biosensor , bacteria , pseudomonadales , degradation (telecommunications) , plasmid , kinetics , catabolism , biodegradation , pseudomonas , biochemistry , enzyme , biophysics , chromatography , biology , gene , gene expression , organic chemistry , telecommunications , genetics , physics , quantum mechanics , computer science
The reporter bacterium, Pseudomonas fluorescens HK44 (HK44), was characterized in an immobilized state to investigate utility for deployment as a remote sensor in the subsurface. A packed‐bed reactor with alginate‐immobilized HK44 simulated hydrodynamic conditions such as might be found in a subsurface environment. The reporter bacterium, HK44, harbors a reporter plasmid, pUTK21, which contains a transcriptional fusion between the nahG gene in the lower pathway of the catabolic plasmic NAH7 and a luxCDABE gene cassette. The upper nah pathway and the lux pathway in pUTK21 are induced by salicylate. The lux enzymes catalyze the light reaction. HK44 demonstrated a quantitative relationship between salicylate concentration and degradation. Light intensity mimicked salicylate concentration, whereas degradation was first order in biomass and first order in salicylate concentration, with a degradation constant of 2.23 × 10 −2 dm 3 g −1 min −1 . © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 54: 491–502, 1997.