Premium
Selective separation of amino acids with a charged inorganic nanofiltration membrane: Effect of physicochemical parameters on selectivity
Author(s) -
Garem A.,
Daufin G.,
Maubois J. L.,
Léonil J.
Publication year - 1997
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19970520)54:4<291::aid-bit1>3.0.co;2-f
Subject(s) - chemistry , nanofiltration , selectivity , membrane , isoelectric point , fractionation , chromatography , amino acid , hydrolysate , ionic strength , organic chemistry , aqueous solution , hydrolysis , biochemistry , catalysis , enzyme
A charged organic‐inorganic nanofiltration (NF) membrane prototype was used to separate a mixture of nine amino acids (AA) on the basis of differential electrostatic interactions with the membrane because, for a given pH, some of them were positively charged, some were negative, and some were zwitterions. Effect of pH, amino acid concentration (C r ), and added ionic strength ([NaCI]) on the process selectivity was studied. A global statistical study revealed that pH was the dominant parameter regarding fractionation. C r and [NaCI] had a weaker effect, but the ratio C r /[NaCI] demonstrated a pronounced effect on system selectivity. Two split‐ups of the mixture were obtained at pH 2 and at pH 12, for a 1‐g/L total AA concentration and a C r /[NaCI] ratio of 0.16. Under these conditions, the differences in transmissions between basic and acid AA were higher than 70%. Interpretation of the results according to the Donnan theory allows us to foresee the potentialities of charged nanofiltration membranes for the fractionation of a complex mixture, such as peptidic hydrolysate to streams containing peptides and amino acids having different isoelectric points. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 54: 291–302, 1997.