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Capture of microbial cells on brush‐type polymeric materials bearing different functional groups
Author(s) -
Lee William,
Saito Kyoichi,
Furusaki Shintaro,
Sugo Takanobu
Publication year - 1997
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19970305)53:5<523::aid-bit10>3.0.co;2-h
Subject(s) - glycidyl methacrylate , membrane , escherichia coli , epoxy , chemistry , diethylamine , monomer , methacrylate , grafting , polymer chemistry , chemical engineering , organic chemistry , polymer , biochemistry , engineering , gene
A brush‐type microbial‐cell‐capturing polymeric material was prepared by radiation‐induced grafting of an epoxy‐group‐containing monomer, glycidyl‐methacrylate (GMA), onto a polyethylene‐based fiber. The epoxy ring (EO) of GMA was opened with different degrees of introduction of diethylamine (DEA). The residual epoxy group was hydrophilized by ethanolamine (EA). The prepared DEA membranes with coexisting EO or EA groups were tested for their ability to capture Staphylococcus aureus and Escherichia coli cells. The DEA membrane (2.7 mol/kg of product of DEA group density) with coexisting EO groups (DEA‐EO membrane) exhibited good S. aureus ‐cell‐capturing ability with a capturing rate constant of 1.82 × 10 −6 m/s, whereas the DEA membrane with coexisting EA groups (DEA‐EA membrane) retarded capturing abilities for both S. aureus and E. coli cells. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 53: 523–528, 1997.