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Intrinsic kinetic parameters of substrate utilization by immobilized anaerobic sludge
Author(s) -
Zaiat Marcelo,
Vieira Lorena Grein Tavares,
Foresti Eugenio
Publication year - 1997
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19970120)53:2<220::aid-bit13>3.0.co;2-o
Subject(s) - substrate (aquarium) , anaerobic exercise , kinetic energy , chemistry , pulp and paper industry , bioreactor , environmental chemistry , chromatography , biochemical engineering , chemical engineering , environmental science , biology , ecology , organic chemistry , engineering , physics , physiology , quantum mechanics
This article presents a method for evaluating the intrinsic kinetic parameters of the specific substrate utilization rate ( r ) equation and discusses the results obtained for anaerobic sludge‐bed samples taken from a horizontal‐flow anaerobic immobilized sludge (HAIS) reactor. This method utilizes a differential reactor filled with polyurethane foam matrices containing immobilized anaerobic sludge which is subjected to a range of feeding substrate flow rates. The range of liquid superficial velocities thus obtained are used for generating data of observed specific substrate utilization rates ( r obs ) under a diversity of external mass transfer resistance conditions. The r obs curves are then adjusted to permit their extrapolation for the condition of no external mass transfer resistance, and the values determined are used as a test for the condition of absence of limitation of internal mass transfer. The intrinsic parameters r max , the maximum specific substrate utilization rate, and K s , the half‐velocity coefficient, are evaluated from the r values under no external mass transfer resistance and no internal mass transfer limitation. The application of such a method for anaerobic sludge immobilized in polyurethane foam particles treating a glucose substrate at 30°C resulted in intrinsic r max and K s , respectively, of 0.330 mg chemical oxygen demand (COD) · mg −1 volatile suspended solids (VSS) · h −1 and 72 mg COD · L −1 . In comparison with the values found in the literature, intrinsic r max is significantly high and intrinsic K s is relatively low. © 1997 John Wiley & Sons, Inc.