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Application of mineral support on cephamycin C production in culture using soybean oil as the sole carbon source
Author(s) -
Mitsuru Ichida,
Park Yong Soo,
Kosakai Yuko,
Okabe Mitsuyasu
Publication year - 1997
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19970120)53:2<207::aid-bit11>3.0.co;2-h
Subject(s) - carbon source , soybean oil , chemistry , carbon fibers , mineral oil , pulp and paper industry , food science , mathematics , organic chemistry , engineering , biochemistry , composite number , algorithm
A mineral support was used for cephamycin C production in a culture using soybean oil as the sole carbon source. When the support was added into an oil‐water system, the soybean oil was emulsified as fine oil droplets, which was observed by a photomicroscope. Mycelia were also twined around the support, which was observed by a scanning electron microscope. That caused the formation of an oil‐mycelia complex on or around the support, which provided a larger specific surface area of oil. When 15 g/L of the support was used in a batch culture of Streptomyces sp. P6621 with 50 g/L of soybean oil, the maximum cephamycin C concentration was 2.8 g/L, which was 2.2 times higher than that without the support. This indicates that the mineral support is useful for the culture system using vegetable oil as a carbon source. © 1997 John Wiley & Sons, Inc.