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Production of antioxidant vitamins, β‐carotene, vitamin C, and vitamin E, by two‐step culture of Euglena gracilis Z
Author(s) -
Takeyama Haruko,
Kanamaru Akihisa,
Yoshino Yuko,
Kakuta Hiroyuki,
Kawamura Yoshiya,
Matsunaga Tadashi
Publication year - 1997
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19970120)53:2<185::aid-bit8>3.0.co;2-k
Subject(s) - carotene , antioxidant , vitamin , food science , carotenoid , beta carotene , vitamin c , retinol , chemistry , vitamin e , biochemistry , biology
Euglena gracilis Z is one of the few microorganisms which simultaneously produces antioxidant vitamins such as β‐carotene and vitamins C and E. Photoheterotrophically cultured E. gracilis Z produced larger levels of biomass but with a lower content of antioxidant vitamins than photoautotrophically grown cultures. For efficient production of these vitamins, a two‐step culture was performed. Cells were grown photoheterotrophically and then transferred to photoautotrophic conditions. When E. gracilis Z cells were grown in fed‐batch culture under photoheterotrophic conditions, their density reached 19 g/L after 145 h. Subsequent transfer of these cells to photoautotrophic conditions increased vitamin content, enhancing the total vitamin yields, which were 71.0 mg/L of β‐carotene, 30.1 mg/L of vitamin E, and 86.5 mg/L of vitamin C. © 1997 John Wiley & Sons, Inc.