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Alginate oligosaccharides as enhancers of penicillin production in cultures of penicillium chrysogenum
Author(s) -
Ariyo Bolatito T.,
Bucke Christopher,
Keshaverz Tajalli
Publication year - 1997
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19970105)53:1<17::aid-bit3>3.0.co;2-1
Subject(s) - penicillium chrysogenum , penicillin , chemistry , food science , yield (engineering) , microbiology and biotechnology , oligosaccharide , hydrolysis , fungi imperfecti , biochemistry , biology , antibiotics , materials science , metallurgy
Oligosaccharide fragments were prepared by partial acid hydrolysis of sodium alginate and consisted of oligomannuronate (OM) and oligoguluronate (OG) blocks. Effects of the OM and OG blocks on penicillin G production by P. chrysogenum were investigated. The oligosaccharides were found to cause significant increases in penicillin G yields. OM blocks at concentrations 10 to 100 μg/mL were used to further evaluate the effects of the oligosaccharides, and were found to enhance the production of penicillin G in shaken flask cultures of P. chrysogenum P2 (high penicillin producer) and NRRL 1951 (low penicillin producer) at the test concentrations. There was an approximately 50% maximum increase in penicillin G yield from biomass in P. chrysogenum P2 cultures and 150% in P. chrysogenum NRRL 1951 cultures, when compared to control cultures without the oligosaccharides. © 1997 John Wiley & Sons, Inc.