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A Fourier‐transform infrared spectroscopic analysis of organic matter degradation in a bench‐scale solid substrate fermentation (composting) system
Author(s) -
Tseng David Y.,
Vir Rahul,
Traina Sam J.,
Chalmers Jeffery J.
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19961220)52:6<661::aid-bit4>3.0.co;2-m
Subject(s) - organic matter , degradation (telecommunications) , substrate (aquarium) , infrared , fourier transform infrared spectroscopy , chemistry , fourier transform , fermentation , scale (ratio) , chemical engineering , materials science , food science , organic chemistry , biology , mathematics , physics , computer science , ecology , engineering , telecommunications , optics , mathematical analysis , quantum mechanics
The degradation of organic matter was evaluated by a quantitative Fourier transform infrared spectroscopy (FTIR) analysis technique. The degradation process was conducted in a bench‐scale reactor under controlled operational conditions of 50°C, with 50–60% moisture content, and subjected to uniform aeration for 325 h. During the composting process, ATP concentration increased from 0.1 to 8 μg/g and the maximum CO 2 evolution and O 2 consumption rates reached 0.04 and 0.085 mmol/g‐h, respectively. Polysaccharide content decreased approximately 50% while lignin content remained unchanged. Three regions of the FTIR spectra were used for quantification: 1070‐974, 1705‐1614, and 2995‐2887 cm −1 , which correspond to polysaccharides and aromatic and aliphatic compounds, respectively. The actual spectra quantification consisted of peak identification using a second derivative and curve fitting technique, followed by normalization using the internal standard CaCO 3 . The results obtained with the spectra quantification technique was then compared to commonly used wet chemistry extraction procedures. Reasonable correlation between the two techniques was obtained. © 1996 John Wiley & Sons, Inc.