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Preparation of 3‐deacetyl cephalosporins by Aspergillus niger lipase
Author(s) -
Carrea Giacomo,
Corcelli Alessandra,
Palmisano Giovanni,
Riva Sergio
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19961220)52:6<648::aid-bit2>3.0.co;2-o
Subject(s) - lipase , aspergillus niger , hydrolysis , chemistry , chromatography , triacylglycerol lipase , organic chemistry , enzyme , biochemistry
Lipase from Aspergillus niger was used for the selective hydrolysis of the 3‐O‐acetate of cephalosporin C to give an intermediate useful for further chemical elaborations. This lipase was purified to homogeneity and its properties compared with previously published data that present some discrepancies. The lipase proved to be very effective in catalyzing 3‐O‐acetate hydrolysis and versatile toward substitution on the β‐lactamic ring. In fact, as an example, two other cephalosporinic derivatives, cephalotin and cefotaxime, were efficiently deacetylated. The lipase was immobilized on Eupergit C and employed continuously in either a column or a batch reactor for 2 months without appreciable loss of activity. © 1996 John Wiley & Sons, Inc.