Premium
CHO DUKX cell lineages preadapted to growth in serum‐free suspension culture enable rapid development of cell culture processes for the manufacture of recombinant proteins
Author(s) -
Sinacore M. S.,
Charlebois T. S.,
Harrison S.,
Brennan S.,
Richards T.,
Hamilton M.,
Scott S.,
Brodeur S.,
Oakes P.,
Leonard M.,
Switzer M.,
Anagnostopoulos A.,
Foster B.,
Harris A.,
Jankowski M.,
Bond M.,
Martin S.,
Adamson S. R.
Publication year - 1996
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19961120)52:4<518::aid-bit7>3.0.co;2-s
Subject(s) - chinese hamster ovary cell , heterologous , recombinant dna , transfection , biology , cell culture , microbiology and biotechnology , heterologous expression , cricetulus , gene , suspension culture , cell growth , biochemistry , genetics
Using an adaptive strategy, Chinese hamster ovary (CHO) cell lines were developed that are capable of robust growth in serum‐free suspension culture. These preadapted derivatives of the commonly used strain of CHO cells (CHO DUKX), termed PA‐DUKX, were used for the introduction and stable expression of several heterologous human genes. A significant advantage of recombinant PA‐DUKX cells was their ability to readily resume growth in serum‐free suspension culture after transfection and amplification of heterologous genes. Expression of recombinant human proteins in PA‐DUKX cells was quantitatively similar to that of lineages generated using conventional CHO DUKX cells. In addition, recombinant human proteins expressed by transfected PA‐DUKX lineages were shown to be biochemically and structurally similar to those expressed in CHO DUKX cells, PA‐DUKX host cell technology provides an opportunity for reducing the time and resources required to develop large‐scale, suspension culture‐based manufacturing processes employing serum‐free medium. © 1996 John Wiley & Sons, Inc.