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New constraints between kinetic parameters explain the (Un)identifiability of enzymatic rate constants
Author(s) -
Straathof A. J. J.,
Heijnen J. J.
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19961105)52:3<433::aid-bit10>3.0.co;2-k
Subject(s) - identifiability , redundancy (engineering) , reaction rate constant , steady state (chemistry) , kinetic energy , thermodynamics , chemistry , biological system , kinetics , mathematics , computer science , physics , statistics , classical mechanics , biology , operating system
For an enzymatic reaction the rate constants in the assumed mechanism ( k 1 , k −1 , etc.) sometimes can be calculated from the steady‐state parameter values ( V max , K m , etc.) and sometimes cannot. When identifiability problems occur, these are obscured by redundancy occurring among the steady‐state parameters. This redundancy is only partly revealed by the known Haldane relations. We found the additional constraints between the parameters. These relations allow to predict in which situation rate constants are identifiable by steady‐state kinetic methods. © 1996 John Wiley & Sons, Inc.