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Two‐dimensional versus three‐dimensional culture systems: Effects on growth and productivity of BHK cells
Author(s) -
Alves P. M.,
Moreira J. L.,
Rodrigues J. M.,
Aunins J. G.,
Carrondo M. J. T.
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19961105)52:3<429::aid-bit9>3.0.co;2-m
Subject(s) - microcarrier , productivity , porous medium , chemical engineering , baby hamster kidney cell , materials science , chemistry , porosity , chromatography , composite material , cell , biochemistry , engineering , economics , macroeconomics
The influence of surface growth (two‐dimensional microcarriers) and three‐dimensional growth (aggregates and macroporous supports) in agitated, suspended batch culture systems upon growth and productivity of BHK was compared. Cultures using three porous microcarriers (CultiSpher G, Cellsnow EX, and Cytocell), one nonporous microcarrier (Cytodex 3) and natural aggregates were performed in stirred tanks using two different agitation rates (60 and 100 RPM). With the exception of Cytocell, cell growth, viability, and productivity were similar when three‐dimensional structures (porous microcarriers and aggregates) were used. Nonporous microcarriers only compared well at 60 RPM as growth ceased under overagitation. These results suggest that cultures less susceptible to fluid shear are advantageous for scale‐up. © 1996 John Wiley & Sons, Inc.