Premium
Effect of oxygen supply on metabolism of immobilized and suspended Escherichia coli
Author(s) -
Inanç Emel,
Miller Judith E.,
DiBiasio David
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19960920)51:6<697::aid-bit8>3.0.co;2-c
Subject(s) - escherichia coli , bioreactor , lactose , chemistry , oxygen , metabolism , chromatography , biochemistry , organic chemistry , gene
The effect of reduced oxygen supply on the production of a recombinant protein (plasmid‐encoded β‐galactosidase) was investigated in Escherichia coli. A novel modified bubble tank reactor was used to provide a direct comparison between immobilized and suspended cells in identical environments except for the immobilization matrix. Decreased oxygen supply led to increased β‐galactosidase synthesis by both immobilized and suspended cells. Immobilized cells produced similar amounts of β‐galactosidase as the suspended cells. Lactose consumption and acetate production, on a per cell basis, were significantly higher in immobilized cells, suggesting that immobilized cells utilized fermentative metabolism. However, a transport analysis of the immobilized cell system showed that immobilized cells were not subject to either external or internal mass transfer gradients.