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Endoplasmic reticulum protein expression in recombinant NS0 myelomas grown in batch culture
Author(s) -
Downham Matthew R.,
Farrell William E.,
Jenkins Helen A.
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19960920)51:6<691::aid-bit7>3.0.co;2-c
Subject(s) - endoplasmic reticulum , recombinant dna , protein biosynthesis , glutamate receptor , biology , amino acid , biochemistry , chemistry , microbiology and biotechnology , gene , receptor
During batch growth of two recombinant NS0 myelomas, an increase in the expression of the endoplasmic reticulum (ER) proteins (GRP78/BiP, GRP94, and ERp72) was observed. A marked increase in these proteins was associated with the decline phase of growth, an increase in the production rate of chimeric antibody, and a marked slowing or halt in the uptake of glucose and glutamate. Refeeding with glucose, glutamate, or a mixture of amino acids just prior to the onset of decline phase failed to repress induction. Although refeeding with glutamate led to an increase in specific productivity, there was no significant difference in the pattern of ER protein induction. These results indicate that an increase in ER protein expression is not solely related to productivity but also to certain changes that occur during the course of batch growth.