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Properties of two insect cell lines useful for the baculovirus expression system in serum‐free culture
Author(s) -
Sugiura Takeyuki,
Amann Egon
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19960820)51:4<494::aid-bit13>3.0.co;2-i
Subject(s) - sf9 , cell culture , sucrose , biology , baculoviridae , glycoprotein , secretion , virus , recombinant dna , ammonia , biochemistry , microbiology and biotechnology , virology , genetics , spodoptera , gene
The properties of Sf9 and Tn5 insect cells were analyzed comparatively under serum‐free culture conditions. Sf9 cells in SF900II medium apparently utilized sucrose as a primary nutrient both before and after virus infection, yielding small amounts of lactate and ammonia. Tn5 cells in Excell 401 medium consumed all the nutrients examined, including sucrose. The productivity of a recombinant glycoprotein, OSF‐2, by Tn5 cells, was moderate in both monolayer and spinner cultures, but the ability to secrete it was compromised in the former case. Relative to the Tn5 cultures, Sf9 produced 30‐fold more OSF‐2 in either culture mode. © 1996 John Wiley & Sons, Inc.