Premium
Optimized enzymatic synthesis of geranyl butyrate with lipase AY from candida rugosa
Author(s) -
Shieh ChwenJen,
Akoh Casimir C.,
Yee Lisa N.
Publication year - 1996
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19960805)51:3<371::aid-bit12>3.0.co;2-p
Subject(s) - candida rugosa , chemistry , tributyrin , substrate (aquarium) , lipase , response surface methodology , chromatography , molar , yield (engineering) , molar ratio , organic chemistry , enzyme , materials science , catalysis , biology , ecology , paleontology , metallurgy
Response surface methodology (RSM) and five‐level, five‐variable central composite rotatable design (CCRD) were used to evaluate the effects of synthetic variables, such as reaction time (1–9 h), temperature (25–65°C), enzyme amount (10–50%), substrate molar ratio of geraniol to tributyrin (1:0.33–1:1), and added water amount (0–20%) on molar percent yield of geranyl butyrate, using lipase AY from Candida rugosa. Reaction time and temperature were the most important variables and substrate molar ratio had no effect on percent molar conversion. Based on contour plots, optimum conditions were: reaction time 9 h, temperature 35°C, enzyme amount 50%, substrate molar ratio 1:0.33, and added water 10%. The predicted value was 100% and actual experimental value was 96.8% molar conversion. © 1996 John Wiley & Sons, Inc.