Premium
Sequential actions of glucose oxidase and peroxidase in molecular films assembled by layer‐by‐layer alternate adsorption
Author(s) -
Onda Mitsuhiko,
Lvov Yuri,
Ariga Katsuhiko,
Kunitake Toyoki
Publication year - 1996
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19960720)51:2<163::aid-bit5>3.0.co;2-h
Subject(s) - glucose oxidase , peroxidase , adsorption , chemistry , layer by layer , layer (electronics) , redox , supramolecular chemistry , polymer chemistry , point of delivery , chemical engineering , ethyleneimine , combinatorial chemistry , enzyme , organic chemistry , molecule , polymer , agronomy , biology , engineering
Molecular films of protein/polyion layers were assembled by means of alternate adsorption through electrostatic interaction. Glucose oxidase (GOD) and peroxidase (POD) were assembled in combination with sodium poly(styrenesulfonate) (PSS) and poly(ethyleneimine) (PEI), respectively. Enzyme activities of those films on specific substrates (glucose and H 2 O 2 ) were examined by coloring reaction of dye DA67. A multienzyme film containing GOD layer and POD layer was prepared by alternate adsorption of POD/PSS followed by PEI/GOD. Sequential redox reaction of glucose/H 2 O 2 /DA67 was demonstrated successfully with this supramolecular system. © 1996 John Wiley & Sons, Inc.