Premium
Surfactant enhancement of ( S )‐naproxen ester productivity from racemic naproxen by lipase in isooctane
Author(s) -
Tsai ShauWei,
Lu ChienCheng,
Chang ChunSheng
Publication year - 1996
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19960720)51:2<148::aid-bit3>3.0.co;2-j
Subject(s) - chemistry , lipase , naproxen , pulmonary surfactant , organic chemistry , chromatography , methanol , triacylglycerol lipase , enzyme , biochemistry , medicine , alternative medicine , pathology
In the enantioselective esterification of racemic Naproxen with trimethylsilyl methanol in isooctane by Candida cylindracea lipase, improvements in ( S )‐naproxen ester productivity and enzyme selectivity were demonstrated by adding bis(2‐ethylhexyl) sodium sulfosuccinate (AOT) as the best surfactant. The effect of water content on the enhancement of enzyme activity was elucidated from the reduced adsorption of surfactant molecules on the lipase. A competitive inhibition by the alcohol and a noncompetitive inhibition by the surfactant to the enzyme were found from the kinetic analysis. By using a two‐phase extraction, a complete separation of the surfactant from the organic solution was obtained. © 1996 John Wiley & Sons, Inc.