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Apparent zero‐order kinetics of phenol biodegradation by substrate‐inhibited microbes at low substrate concentrations
Author(s) -
Shishido Masahiro,
Toda Masayuki
Publication year - 1996
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19960620)50:6<709::aid-bit12>3.0.co;2-9
Subject(s) - biodegradation , substrate (aquarium) , kinetics , phenol , chemistry , zero order , environmental chemistry , substrate specificity , microbiology and biotechnology , first order , biology , biochemistry , ecology , organic chemistry , enzyme , mathematics , physics , quantum mechanics
The reaction kinetics for phenol biodegradation at low substrate concentrations can be estimated based on the analysis of changes in the dissolved oxygen concentration in the bulk liquid during biodegradation. The measured oxygen concentration changes with an interesting behavior as biodegradation proceeds. The oxygen concentration in the bulk liquid decreases rapidly in the early stages of degradation and subsequently decreases linearly and then rapidly recovers to the initial saturated level. Taking into account the oxygen transfer rate between gas and liquid phases and oxygen consumption rate by microbes, the change in the dissolved oxygen concentration can be simulated with an unsteady state mass balance equation and three kinetic models for the rate of phenol metabolism: a substrate‐inhibited model; a zero‐order model; and a combined model. In the combined model, it is assumed that, at phenol concentrations above 10 mg/L, the degradation rate is expressed by a substrate‐inhibited model; whereas at concentrations below 10 mg/L the zero‐order model is applied. It was found that the characteristics of the change in the dissolved oxygen concentration, especially the rapid increase at the end of degradation, can only be described by the combined kinetic model. This result suggests that conventional Haldane‐type kinetics would be unsuitable for estimating the phenol consumption rate at low phenol concentrations, in particular, at concentrations less than 10 mg/L. © 1996 John Wiley & Sons, Inc.