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Effect of culture age on the susceptibility of differentiating neuroblastoma cells to retinoid cytotoxicity
Author(s) -
Kisaalita William S.,
Bowen John M.
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19960605)50:5<580::aid-bit13>3.0.co;2-n
Subject(s) - retinoid , retinoic acid , cytotoxicity , isotretinoin , neuroblastoma , biology , tretinoin , in vivo , clone (java method) , in vitro , cell culture , microbiology and biotechnology , pharmacology , chemistry , biochemistry , genetics , dna , acne
The cytotoxic effects of retinoids on neuroblastoma cells at various times during electrophysiological differentiation were evaluated. We used N1E‐115, a clone of the murine neuroblastoma C1300 derived from the neural crest, and three retinoids: vitamin A (retinol), all‐trans retinoic acid (tretinoin), and 13‐cis‐retinoic acid (isotretinoin). Differentiating N1E‐115 cells exposed to retinoids at an isotretinoin EC 50 of 16 μ M exhibited the greatest vulnerability in terms of cell death during a period (8 to 10 days) that was previously found to be the most sensitive for induction of gross malformations in rodents. This finding suggested possible similarities between the in vivo and in vitro retinoid mechanism(s) of action. The greatest period of vulnerability to retinoid cytotoxicity was also found to coincide with the rapid resting membrane potential ( V m ) development period, suggesting a linkage between neuronal V m and/or electrical excitability development and vulnerability to retinoid cytotoxicity. © 1996 John Wiley & Sons, Inc.