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Increased PHB productivity by high‐cell‐density fed‐batch culture of Alcaligenes latus , a growth‐associated PHB producer
Author(s) -
Yamane Tsuneo,
Fukunaga Masaaki,
Lee Yong Woo
Publication year - 1996
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19960420)50:2<197::aid-bit8>3.0.co;2-h
Subject(s) - fed batch culture , food science , ammonia , chemistry , productivity , sucrose , polyhydroxybutyrate , dry weight , nitrogen , biology , biochemistry , botany , bacteria , organic chemistry , fermentation , genetics , economics , macroeconomics
Alcaligenes latus , a growth‐associated PHB producer, was cultivated by a pH‐stat modal fed‐batch culture technique to attain high PHB productivity. Both sucrose solution and inorganic medium were fed in conjunction with the supply of ammonia solution which serves as a nitrogen source and as a means of pH control. Compositions of the inorganic medium were formulated by elemental analysis of A. latus cell mass. The effect on inoculum size was examined to reduce culture time. High concentrations of cell (142 g/L) and PHB (68.4 g/L) were obtained in a short culture time (18 h) with an inoculum size of 13.7 g/L. The PHB content and the PHB productivity at the end of the fed‐batch culture were 50% of dry cell weight and 4.0 g PHB/(L · h), respectively. © 1996 John Wiley & Sons, Inc.