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Modification of native collagen reduces antigenicity but preserves cell compatibility
Author(s) -
HardinYoung Janet,
Carr Robert M.,
Downing Gregory J.,
Condon Kimberlie D.,
Termin Paul L.
Publication year - 2000
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/(sici)1097-0290(19960320)49:6<675::aid-bit9>3.0.co;2-l
Subject(s) - carbodiimide , chemistry , antigenicity , antibody , immune system , antigen , microbiology and biotechnology , humoral immunity , biochemistry , immunology , biology
Porcine intestinal collagen (ICL), derived from processed small intestine, is used as a part of a remodelable bilaminate biosynthetic vascular prosthesis. The process for the production of ICL involves mechanical cleaning of non‐crosslinked porcine intestine (NC‐ICL), disinfection with peracetic acid (PA‐ICL), and crosslinking with 1‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide hydrochloride (PA/EDC‐ICL). Two model systems were investigated to evaluate the effect of these agents on the humoral response to NC‐ICL. First, the antibody titers of rabbits immunized with NC‐ICL, PA‐ICL, and PA/EDC‐ICL were determined, and second, the humoral response of canines receiving collagenous vascular implants was examined. Collagenous and noncollagenous fractions were extracted from NC‐ICL, PA‐ICL, and PA/EDC‐ICL and separated by SDS‐PAGE. PA and EDC treatment decreased the number of extractable proteins as compared to NC‐ICL. Immunoblot techniques demonstrated anti‐NC‐ICL antibodies recognized multiple immunoreactive proteins in NC‐ICL, but not in PA‐ICL or PA/EDC‐ICL; and rabbits immunized with NC‐ICL produced higher antibody titers to ICL proteins than rabbits immunized with either PA‐ICL or PA/EDC‐ICL. It was, therefore, apparent that NC‐ICL was more antigenic than either PA‐ICL or PA/EDC‐ICL. The humoral immune response of canines to PA/EDC‐ICL fabricated vascular grafts was determined. At 4 weeks, 8 weeks, and 12 weeks postimplant, serum antibodies to ICL proteins or type I collagen could not be detected. These data demonstrate a reduced humoral immune response to PA/EDC‐ICL. © 1996 John Wiley & Sons, Inc.